Fibrina inyectable con ácido hialurónico como sistema de liberación intradérmica. Ensayo preclínico en modelo murino

Dubraska Suárez, Gladys Velazco

Resumen


INTRODUCCIÓN: En armonización orofacial los sistemas autólogos inyectables para liberación intradérmica (SLC) son una innovación. Podrían cargarse con activos como aminoácidos, o ácido hialurónico para potenciar su acción. El ácido hialurónico (AH) de baja reticulación se ha valorado ampliamente en la reestructuración cutánea, pero no hay evidencia que demuestre sus efectos cutáneos cuando se le administra en un SLC de fibrina rica en plaquetas inyectable (iPRF). OBJETIVO: Evaluar histomorfométricamente la efectividad del SLC iPRF- AH versus AH en la reestructuración cutánea. METODO: Se administraron vía intradérmica los tratamientos en 13 ratas BIOU Wistar divididas en grupo control-control (3), grupo experimental iPRF + AH (5) y grupo control AH (5). A las 4 semanas se obtuvieron muestras cutáneas para ulterior análisis histológico. Los hallazgos fueron analizados mediante estadística descriptiva en el SPSS V19. RESULTADOS: El AH, no mostró infiltrado inflamatorio, cambios celulares reactivos, ni reacción inflamatoria a cuerpo extraño. Mostró escasos folículos pilosos (80%) y vasos sanguíneos (20%), tejido conectivo laxo (80%) con fibras colágenas tipo I separadas (60%) y algunas fragmentadas (20%), escaso edema intercelular (60%), número de fibroblastos moderado (80%), epidermis delgada con membrana basal ondulante (100%)y grosor dérmico moderado (80%) . En iPRF+AH tampoco se observó infiltrado inflamatorio, reacción a cuerpo extraño o cambios celulares reactivos. Mostró escasos vasos sanguíneos (20%), escasos folículos pilosos (100%), tejido conectivo laxo con fibras colágenas delgadas y fragmentadas (80%), moderado volumen intercelular (80%), celularidad de fibroblastos moderada (60%), epidermis delgada con membrana basal ondulante (100%) y grosor dérmico delgado (60%). CONCLUSIONES: El iPRF+ AH genera menor bioestimulación de los fibroblastos que AH control, por lo que la mejor calidad de las fibras colágenas fue observada con el AH sin conjugar. iPRF+ AH conduce a un considerable mayor volumen intercelular, benéfico para propósitos de hidratación pues no está asociado a respuesta inflamatoria o cambios celulares. El SLC iPRF+AH evita la respuesta inflamatoria tardía y restringe la alopecia observada al administrar AH. Se recomienda estudiar el comportamiento del sistema iPRF +AH en modelos envejecimiento cutáneo avanzado.

Recibido: 07-01-23 Aceptado: 01-02-23

Palabras clave


Ácido Hialurónico; Fibrina rica en plaquetas inyectables; Sistemas de liberación controlada; Sistemas de liberación transdérmica; Reestructuración cutánea

Texto completo:

PDF

Referencias


Fitzgerald R, Graivier MH, Kane M, Lorenc ZP, Vleggaar D, Werschler WP, Kenkel JM. Update on facial aging. Aesthet Surg J. 2010; 30 Suppl: 11S-24S. DOI: 10.1177/1090820X10378696.

Liu S, Chen Z, Cai X, Sun Y, Zhao C, Liu F, Liu D. Effects of dimethylaminoethanol and compound amino acid on D- galactose induced skin aging model of rat Scientific World Journal. 2014; 2014:507351. 7 pages. http://dx.doi.org/10.1155/2014/507351.

Murakami H, Shimbo K, Inoue Y, Takino Y, Kobayashi H. Importance of amino acid composition to improve skin collagen protein synthesis rates in UV-irradiated mice. Amino Acids. 2012; 42:2481–2489. DOI: 10.1007/s00726-011- 1059-z

D. Vasile, R. Iancu, C. Bogdanici, E. Ungureanu, D. Ciobotea, R. Ciuluvica, G. Iancu,

Chemophysical Properties and Biomedical Applications of Hyaluronic Acid in Medicine, Revista De Chimie 2017; 68(2) 384-386.

A. Fakhari, C. Berkland, Applications and emerging trends of hyaluronic acid n tissue engineering, as a dermal filler and in osteoarthritis treatment, Acta biomaterialia 2013; 9(7): 7081-7092

G. Agarwal, S. Agiwal and A. Srivastava, Hyaluronic acid containing scaffolds ameliorate stem cell function for tissue repair and regeneration, International Journal of Biological Macromolecules. 2018. https://doi.org/10.1016/j.ijbiomac.2020.09.107

Kogan Grigorij, Šoltés Ladislav, Stern Robert, Schiller Jürgen, Mendichi Raniero. Hyaluronic Acid: Its Function and Degradation in in vivo Systems. Studies in Natural Products Chemistry 2008; 34: 789-882. DOI: 10.1016/S1572- 5995(08)80035-X.

A. Passi, D. Vigetti, Hyaluronan: Structure, Metabolism, and Biological Properties, Extracellular Sugar-Based Biopolymers Matrices, Springer2019, pp. 155-186

Velazco de Maldonado GJ, Suárez-Vega DV, García-Guevara V, Miller-Kobisher B. Innovative Paradigm in Aesthetics Medicine: Proposal for Diagnostic MorphologicalGeometric by Thirds, Semiology in Clinical Applied to Aging Facial. J Cutan Aesthet Surg.

Apr-Jun; 13(2):112-123. DOI: 10.4103/JCAS.JCAS_116_19. PMID: 32792772; PMCID: PMC7394105.

Suárez D, 2020. La ritidoreversión. Un fenómeno predecible. Editorial. Acta Bioclínica. Julio-Diciembre. 2021

M.A. Solis, Y.-H. Chen, T.Y. Wong, V.Z. Bittencourt, Y.-C. Lin, L.L. Huang, Hyaluronan regulates cell behavior: a potential niche matrix for stem cells, Biochemistry research international 2012 (2012) 346972.

J. Lam, N.F. Truong, T. Segura, Design of cell–matrix interactions in hyaluronic acid hydrogel scaffolds, Acta biomaterialia. 2014; 10(4): 1571-1580.

L. Liu, A Ballada A. Engineering of Polymers and Chemical Complexity: CurrentState of the Art and Perspectives: First Edition, Apple Academic Press; United State, 2014.

C. Chircov, A.M. Grumezescu, L.E. Bejenaru, Hyaluronic acid-based scaffolds for tissue engineering, Rom. J. Morphol. Embryol 2018; 59 71-76.

Choi SW, Pangeni R, Jung DH, Kim SJ, Park JW. Construction and characterization of cell-penetrating peptide-fused fibroblast growth factor and vascular endothelial growth factor for an enhanced percutaneous delivery system. J Nanosci Nanotechnol. 2018; 18:842- 847.

Choukroun J, Ghanaati S (Eds). Introducing the Low-Speed Centrifugation Concept. In Platelet Rich Fibrin in Regenerative Dentistry: Biological Background and Clinical Indications: Biological Background and Clinical Indications. First Ed. 2017. John Wiley & Sonshttps://doi.org/10.1002/9781119406792.ch3

Anitua E. La utilización de los factores de crecimiento plasmáticos en cirugía oral, maxilofacial y periodoncia (PRGF). RCOE. 2001; 6(3).

Andreone A and Hollander D. A Retrospective Study on the Use of Dermis

Micrografts in Platelet-Rich Fibrin for the Resurfacing of Massive and Chronic Full-N Thickness Burns. Stem Cells International .Volume 2019, Article ID 8636079, 9 pages

https://doi.org/10.1155/2019/8636079

Dohle E, El Bagdadi K, Sader R, Choukroun J, James Kirkpatrick C, Ghanaati S. Platelet-rich fibrin-based matrices to improve angiogenesis in an in vitro co-culture model for bone tissue engineering. J Tissue EngRegen Med. 2018;12:598-610

Miron RJ, Zucchelli G, Pikos MA, et al. Use of platelet-rich fibrin in regenerative dentistry: a systematic review. Clin Oral Investig. 2017; 21:1913-1927

Kubesch A, Barbeck M, Al-Maawi S, et al. A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich ibrin: an experimental study in vivo. Platelets. 2018; 6:1-12.

Nacopoulos C1, Vesala AM. Lower facial regeneration with a combination of platelet-rich fibrin liquid matrices based on the low speed centrifugation concept-Cleopatra technique. J Cosmet Dermatol. 2019. DOI: 10.1111/jocd.13196.

Kim DH, Je YJ, Kim CD, et al. Can platelet-rich plasma be used for skin

rejuvenation? Evaluation of effects of platelet- rich plasma on human dermal fibroblast. Ann Dermatol. 2011; 23:424-431. 3.

Redaelli A. Face and neck revitalization with platelet-rich plasma (PRP): clinical outcome in a series of 23 consecutively treated patients. J Drugs Dermatol. 2010; 9:466-472.

Sclafani AP. Platelet-rich fibrin matrix for improvement of deep nasolabial folds. J Cosmet Dermatol. 2010; 9:66-71. 5.

De Almeida C, Valiense H, Rodriguez E, N M, Mourao F, Calasans M. Obtention of njectable platelets rich-fibrin (i- PRF) and its polymerization with bone graft: technical note. Rev Col Bras Cir. 2015; 42(6).

Yao Xue Xue Bao. Advances in the study of the rat model of aging induced by D-

galactose. Acta pharmaceutica Sinica 2017; 52(3):347-354

Fitzgerald R, Graivier MH, Kane M, Lorenc ZP, Vleggaar D, Werschler WP, Kenkel JM. Update on facial aging. Aesthet Surg J. 2010; 30 Suppl: 11S-24S. DOI: 10.1177/1090820X10378696.

Kema VH, Khan I, Jamal R, Vishwakarma SK, Lakki Reddy C, Parwani K, Patel F, Patel D, Khan AA, Mandal P.Protective Effects of Diallyl Sulfide Against Ethanol-Induced

Un7jury in Rat Adipose Tissue and Primary Human Adipocytes. Alcohol Clin Exp Res. 2017;

(6):1078-1092. 30.

Jankovic A, Saso L, Korac A, Korac BRelation of Redox and Structural Alterations of Rat Skin in the Function of Chronological Aging. Oxidative Medicine and Cellular Longevity. Volume 2019, Article

ID

,

pages,

https://doi.org/10.1155/2019/2471312

Lynch B, Bonod-Bidaud C, Ducourthial G, Affagard JS, Bancelin S,

Psilodimitrakopoulos S, Ruggiero F, Allain JM & Schanne-Klein MC. How aging impacts skin biomechanics: a multiscale study in mice. Scientific Reports 2017; 7: 13750. DOI:10.1038/s41598-017-13150-4

Csoka AB, Stern R. 2013. Hypotheses on the evolution of hyaluronan: a highly ironic acid. Glycobiology 23:398-411

Fernández-Martos S, Calvo-Sánchez M, García-Alonso K, Castro B, Hashtroody B, Espada J. Sustained Human Hair Follicle Growth Ex Vivo in a Glycosaminoglycan Hydrogel Matrix. Int J Mol Sci. 2019 Apr 9;20(7):1741. DOI: 10.3390/ijms20071741. PMID: 30970537; PMCID: PMC6480973.

Asz-Sigall D, Iñigo-Gomez K, Ortega-Springall MF, Vega-Memije ME, Arenas R,Tosti A. Alopecia Secondary to Hyaluronic Acid Embolization: Trichoscopic Findings. Skin Appendage Disord. 2019 Nov;5(6):396-400. doi: 10.1159/000502262. Epub 2019 Sep 18.

PMID: 31799272; PMCID: PMC6883453.

Ulusal BG. Platelet-rich plasma and hyaluronic acid - an efficient biostimulation method for face rejuvenation. J Cosmet Dermatol. 2017; 16(1):112-119. doi: 10.1111/jocd.12271. Epub 2016 Sep 5. PMID: 27595866.

Pierre-Antoine Deglesne, Rodrigo Arroyo, Evgeniya Ranneva, Philippe Deprez. Estudio in vitro del producto de mesoterapia / biorevitalización inyectable RRS HA en fibroblastos de piel humana y su utilización clínica. Clinical, Cosmetic and Investigational Dermatology 2016:9 41–53.

de Melo BAG, França CG, Dávila JL, Batista NA, Caliari-Oliveira C, d'Ávila MA, Luzo ÂCM, Lana JFSD, Santana MHA. Hyaluronic acid and fibrin from L-PRP form semi-IPNs with tunable properties suitable for use in regenerative medicine. Mater Sci Eng Mater Biol Appl. 2020; 109:110547. DOI: 10.1016/j.msec.2019.110547. Epub 2019 Dec 13.

PMID: 32228935

Scarano A, Sbarbati A, Amore R, Iorio EL, Ferraro G, Marchetti M, Amuso D. The role of hyaluronic acid and amino acid against the aging of the human skin: A clinical and histological study. J Cosmet Dermatol. 2020 Oct 22. DOI: 10.1111/jocd.13811. Epub ahead of print. PMID: 33090687.

Ciccone V, Zazzetta M, Morbidelli L. Comparison of the Effect of Two HyaluronicAcid Preparations on Fibroblast and Endothelial Cell Functions Related to Angiogenesis.

De Angelis B, D'Autilio MFLM, Orlandi F, Pepe G, Garcovich S, Scioli MG, Orlandi A, Cervelli V, Gentile P. Wound Healing: In Vitro and In Vivo Evaluation of a Bio-Functionalized Scaffold Based on Hyaluronic Acid and Platelet-Rich Plasma in Chronic Ulcers. J Clin Med. 2019; 8(9):1486. doi: 10.3390/jcm8091486. PMID: 31540446; PMCID: PMC6780765

Afat İM, Akdoğan ET, Gönül O. Effects of Leukocyte- and Platelet-Rich Fibrin Alone and Combined With Hyaluronic Acid on Pain, Edema, and Trismus after Surgical Extraction of Impacted Mandibular Third Molars. J Oral Maxillofac Surg. 2018 May;76(5):926-932. DOI: 10.1016/j.joms.2017.12.005. Epub 2017 Dec 13. PMID: 29304325.

Martins Shimojo AA, Santos Duarte ADS, Santos Duarte Lana JF, Malheiros Luzo ÂC, Fernandes AR, Sanchez-Lopez E, Barbosa Souto E, Andrade Santana MH. Association of Platelet-Rich Plasma and Auto-Crosslinked Hyaluronic Acid Microparticles: Approach for Orthopedic Application. Polymers (Basel). 2019; 11(10):1568. DOI:

3390/polym11101568. PMID: 31561615; PMCID: PMC6835642

f

Russo F, D'Este M, Vadalà G, Cattani C, Papalia R, Alini M, Denaro V. Platelet Rich Plasma and Hyaluronic Acid Blend for the Treatment of Osteoarthritis: Rheological and Biological Evaluation. PLoS One. 2016; 11(6):e0157048. DOI:10.1371/journal.pone.0157048. PMID: 27310019; PMCID: PMC4911091.

Zhao J, Huang H, Liang G, Zeng LF, Yang W, Liu J. Effects and safety of thecombination of platelet-rich plasma (PRP) and hyaluronic acid (HA) in the treatment of kneeosteoarthritis: a systematic review and meta-analysis. BMC Musculoskelet Disord. 2020; 21(1):224. DOI: 10.1186/s12891-020-03262-w. PMID: 32278352; PMCID: PMC7149899.

Iio K, Furukawa KI, Tsuda E, Yamamoto Y, Maeda S, Naraoka T, Kimura Y, Ishibashi Y. Hyaluronic acid induces the release of growth factors from platelet-rich plasma. Asia Pac J Sports Med Arthrosc Rehabil Technol. 2016; 4:27-32. DOI: 10.1016/j.asmart.2016.01.001. PMID: 29264260; PMCID: PMC5730656.

Jankovic A, Saso L, Korac A, Korac BRelation of Redox and Structural Alterations

of Rat Skin in the Function of Chronological Aging. Oxidative Medicine and Cellular

Longevity.

Volume

,

Article

ID

,

pages,

https://doi.org/10.1155/2019/2471312




Creative Commons License
Todos los documentos publicados en esta revista se distribuyen bajo una
Licencia Creative Commons Atribución -No Comercial- Compartir Igual 4.0 Internacional.
Por lo que el envío, procesamiento y publicación de artículos en la revista es totalmente gratuito.