Estudio comparativo ultraestructural del plasma rico en plaquetas obtenido mediante centrifugación individual y doble centrifugación
Resumen
La regeneración tisular ha sido uno de los retos más anhelados en la odontología. Debido a esto se han desarrollado gran cantidad debiomateriales, entre ellos el Plasma Rico en Plaquetas (PRP). Este es un producto biológico, autólogo, no tóxico ni alergénico quecontiene las plaquetas en forma de sobrenadante tras la centrifugación de sangre anticoagulada. El objetivo de este trabajo fuecomparar, ultraestructuralmente, la morfología de las plaquetas en el PRP obtenido mediante el centrifugado individual y doblecentrifugación, utilizando microscopia electrónica de trasmisión (MET). Mediante observación estructurada, las muestras sanguíneasfueron obtenidas de 5 pacientes de ambos sexos. A cada individuo se le extrajo una muestra de 20 ml, que se dividió en dos partes. Lasmuestras pasaron por separado por cada uno de los procesos de centrifugación. Luego de ser preparadas, se observaron al MET en elCentro de Microscopia Electrónica "Dr. Ernesto Palacios Prü” de la Universidad de Los Andes. Los resultados obtenidos indicaronalteración de la ultraestructura plaquetaria en ambos métodos, con mayor daño al ser sometidas al método de doble centrifugaciónespecíficamente en las organelas, respecto al método de centrifugación individual. La ultraestructura plaquetaria puede verse afectadaluego de ser sometida a procesos de centrifugación para la obtención de PRP con menos daño al utilizar el método de centrifugaciónindividual.
Comparative study of ultrastructural platelet rich plasma obtained by single and Double centrifugation
Abstract
Tissues regeneration has been one of the most expected challenges in dentistry. Due those expectations great deals of biomaterialssuch as the Platelet Rich Plasma (PRP) have been developed. PRP is a non-toxic, non-allergic, autologous, biological productcontaining platelets as a supernatant after the centrifugation of anticoagulated blood. The aim of the present study was toultrastructurally compare, by using Transmission Electron Microscopy (TEM), the morphology of platelets in PRP obtained throughsingle and double centrifugation. Through structured observations, blood samples were obtained from five patients both, female andmale. A 20 ml sample was taken from each individual. Later, those samples were divided in 2 parts and each was separately submittedto one centrifugation condition (single and double). After prepared, those samples were observed by using TEM at the ElectronicMicroscopy Center “Dr. Ernesto Palacios Prü” from the University of Los Andes. Results showed alteration in the plateletsultrastructure for samples treated with both methods, but there was more damage, specifically on the organelles in samples of doublecentrifugation when compared to single centrifugation ones. The platelets ultrastructure may be affected after being submitted tocentrifugation processes to obtain PRP, but less damage is present when using the single centrifugation method.
Comparative study of ultrastructural platelet rich plasma obtained by single and Double centrifugation
Abstract
Tissues regeneration has been one of the most expected challenges in dentistry. Due those expectations great deals of biomaterialssuch as the Platelet Rich Plasma (PRP) have been developed. PRP is a non-toxic, non-allergic, autologous, biological productcontaining platelets as a supernatant after the centrifugation of anticoagulated blood. The aim of the present study was toultrastructurally compare, by using Transmission Electron Microscopy (TEM), the morphology of platelets in PRP obtained throughsingle and double centrifugation. Through structured observations, blood samples were obtained from five patients both, female andmale. A 20 ml sample was taken from each individual. Later, those samples were divided in 2 parts and each was separately submittedto one centrifugation condition (single and double). After prepared, those samples were observed by using TEM at the ElectronicMicroscopy Center “Dr. Ernesto Palacios Prü” from the University of Los Andes. Results showed alteration in the plateletsultrastructure for samples treated with both methods, but there was more damage, specifically on the organelles in samples of doublecentrifugation when compared to single centrifugation ones. The platelets ultrastructure may be affected after being submitted tocentrifugation processes to obtain PRP, but less damage is present when using the single centrifugation method.
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