Envíe este artículo por correo electrónico 
La curcumina mitiga el estrés oxidativo y la hepatotoxicidad inducidos por el glutamato monosódico mediante la alteración de la expresión del gen Nrf2 en ratas albinas Wistar
Resumen
El glutamato monosódico (GMS) es la sal sódica del ácido glutámico, comúnmente utilizado como potenciador del sabor. A pesar de su capacidad para estimular el gusto y aumentar el apetito, los informes indican que el GMS es tóxico para humanos y animales de experimentación. Se ha demostrado que la exposición crónica al GMS en dosis bajas induce estrés oxidativo, por lo que es fundamental encontrar un agente hepatoprotector eficaz. Este estudio evalúa los efectos hepatoprotectores y antioxidantes de la curcumina (CUR) contra el estrés oxidativo inducido por el GMS en ratas. Las ratas del grupo I recibieron carboximetilcelulosa (CMC) al 0,7 % por vía oral y sirvieron como control. Las ratas del grupo II recibieron GMS (4 mg/kg de peso corporal por vía oral). Los grupos III y IV recibieron GMS seguido de CUR y silimarina (SIL), ambas a 100 mg/kg de peso corporal por vía oral, respectivamente. Los grupos V y VI se trataron solo con CUR y SIL. Todos los tratamientos se administraron durante 60 días. Las enzimas marcadoras de hepatotoxicidad AST, ALT y ALP se estimaron en suero y tejido hepático. Los indicadores de estrés oxidativo (LPO, SOD, CAT, GSH, GPX, GR, GST), ATPasa unida a membrana (Na2+ATPasa, Mg2+ATPasa y Ca2+ATPasa) e histopatología se evaluaron en tejido hepático. Se utilizaron transferencia Western e inmunocitoquímica para determinar los niveles de expresión del factor nuclear eritroide 2 relacionado con el factor (Nrf2). Los resultados revelaron que las alteraciones significativas en todos los marcadores de hepatotoxicidad, estrés oxidativo, enzimas unidas a membrana y expresión del gen Nrf2 causadas por dosis bajas de MSG en exposición crónica se mitigaron eficazmente mediante el tratamiento con CUR, lo que indica sus propiedades hepatoprotectoras y antioxidantes.
Recibido: 27/01/2025
Aceptado: 09/03/2025
Palabras clave
Texto completo:
PDF (English)Referencias
Zanfirescu, A., Ungurianu, A., Tsatsakis, A. M., Ni?ulescu, G. M., Kouretas, D., Veskoukis, A.,Margina, D. A review of the alleged health hazards of monosodium glutamate. Comprehensive reviews in food science and food safety, 18(2019)., 1111.
Kazmi, Z., Fatima, I., Perveen, S., & Malik, S. S. Monosodium glutamate: Review on clinical reports. International Journal of food properties, 20(2017), 1807.
Ataseven, N., Yüzbasioglu, D., Keskin, A. Ç., & Ünal, F. Genotoxicity of monosodium glutamate. Food and Chemical Toxicology, 91(2016), 8.
Freeman, M. Reconsidering the effects of monosodium glutamate: a literature review. Journal of the American Association of Nurse Practitioners, 18(2006),482.
Nayanatara, A. K., Vinodini, N. A., Damodar, G., Ahemed, B., Ramaswamy, G. R., Shabarianth, S., & Ramesh, B. M. Role of ascorbic acid in monosodium glutamate mediated effect on testicular weight, sperm morphology and sperm count, in rat testis (2008).
Onyema, O. O., Farombi, E. O., Emerole, G. O., Ukoha, A. I., & Onyeze, G. O. Effect of vitamin E on monosodium glutamate induced hepatotoxicity and oxidative stress in rats(2006).
Hajihasani, M. M., Soheili, V., Zirak, M. R., Sahebkar, A., & Shakeri, A. Natural products as safeguards against monosodium glutamate-induced toxicity. Iranian journal of basic medical sciences, 23(2020), 416.
Kayode, O. T., Bello, J. A., Oguntola, J. A., Kayode, A. A. A., & Olukoya, D. K. The interplay between monosodium glutamate (MSG) consumption and metabolic disorders. Heliyon(2023).
Nandi, A., Yan, L.-J., Jana, C. K., & Das, N. Role of catalase in oxidative stress-and age-associated degenerative diseases. Oxidative Medicine and Cellular Longevity, 1(2019), 9613090.
Memarzia, A., Khazdair, M. R., Behrouz, S., Gholamnezhad, Z., Jafarnezhad, M., Saadat, S., & Boskabady, M. H. Experimental and clinical reports on anti-inflammatory, antioxidant, and immunomodulatory effects of Curcuma longa and curcumin, an updated and comprehensive review. Biofactors, 47(2021), 311-350.
Fuloria, S., Mehta, J., Chandel, A., Sekar, M., Rani, N. N. I. M., Begum, M. Y., Nordin, R. A comprehensive review on the therapeutic potential of Curcuma longa Linn. in relation to its major active constituent curcumin. Frontiers in Pharmacology, 13(2022), 820806.
Gull, N., Arshad, F., Naikoo, G. A., Hassan, I. U., Pedram, M. Z., Ahmad, A., . . . Charbe, N. Recent advances in anticancer activity of novel plant extracts and compounds from Curcuma longa in hepatocellular carcinoma. Journal of Gastrointestinal Cancer, 54(2023), 368.
Flora K, Hahn M, Rosen H, Benner K. Milk thistle (Silybum marianum) for the therapy of liver disease. Am J Gastroenterol. 93(1998),139.
Gillessen A, Herrmann WA, Kemper M, Morath H, Mann K. Effect of silymarin on liver health and quality of life. Results of a non-interventional study. MMW Fortschr Med.;156(2014):120.
Chun, K.S., Raut, P.K., Kim, D.H., Surh, Y.J., 2021. Role of chemopreventive phytochemicals in NRF2-mediated redox homeostasis in humans. Free. Radic. Biol. Med 172(2021),699.
He, W.J., Lv, C.H., Chen, Z., Shi, M., Zeng, C.X., Hou, D.X., et al., 2023. The Regulatory Effect of Phytochemicals on Chronic Diseases by Targeting Nrf2-ARE Signaling Pathway. Antioxidants. (basel) 12(2023),3390.
Rania M. Khalila Naglaa F. Khedrb ,Curcumin Protects against Monosodium Glutamate Neurotoxicity and Decreasing NMDA2B and mGluR5 Expression in Rat Hippocampus ,Neurosignals. 2016;24:81-87
Shatadal Ghosh, Sudip Bhattacharyya, Kahkashan Rashid, Parames C. Sil, Curcumin protects rat liver from streptozotocin-induceddiabetic pathophysiology by counteracting reactive oxygenspecies and inhibiting the activation of p53 and MAPKsmediated stress response pathways, Toxicology Reports 2 (2015) 365–376.
Mohamed E. Shakera, Khaled R. Zalatab, Wajahat Z. Mehalc, Gamal E. Shihad, and Tarek M.Ibrahim, Comparison of imatinib, nilotinib and silymarin in the treatmentof carbon tetrachloride-induced hepatic oxidative stress, injury and fibrosis. Toxicol Appl Pharmacol. 2011 April 15; 252(2): 165–175.
Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol. ;28(1957):56.
King J. The phosphohydrolases - acid and alkaline phosphatases. In: Practical clinical enzymology, D. Van Nostrand Co Ltd., London, (1965)a:191.
Ohkawa H, Ohishi N & Yagi K, Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem,95 (1979) 351.
Ellman, George L. "Tissue Sulfhydryl Groups." Archives of Biochemistry and Biophysics 82, no. 1 (1959): 70.
Beutler, Ernest, et al. "Improved Method for the Determination of Blood Glutathione." Journal of Laboratory and Clinical Medicine, 61 (1963) 882.
Habig, W. H., M. J. Pabst, and W. B. Jakoby. "Glutathione S-transferases: The First Enzymatic Step in Mercapturic Acid Formation." Journal of Biological Chemistry 249, (1974): 7130.
Marklund, Stig, and Göran Marklund. "Involvement of the Superoxide Anion Radical in the Autoxidation of Pyrogallol and a Convenient Assay for Superoxide Dismutase." European Journal of Biochemistry, 47, (1974), 469.
Sinha, A. K. "Colorimetric Assay of Catalase." Analytical Biochemistry 47, (1972): 389.
Rotruck, John T., A. L. Pope, H. E. Ganther, A. B. Swanson, D. G. Hafeman, and W. G. Hoekstra. "Selenium: Biochemical Role as a Component of Glutathione Peroxidase." Science 179, (1973), 588.
Mize, Charles E., and R. G. Langdon. "Hepatic Glutathione Reductase." Journal of Biological Chemistry 237, (1962), 2583.
Omaye, Stanley T. "Effects of Vitamin E on Exercise-Induced Oxidative Stress." Journal of Applied Physiology 46, (1979), 765.
Varley, Hugh, A. H. Gowenlock, and M. Bell. Practical Clinical Biochemistry. London: William Heinemann Medical Books, 1976.
Bonting, Sjoerd L. "Mechanisms of Sodium Transport." Physiological Reviews 50, (1970): 559.
Ohnishi, Tomoko, H. Yamazaki, T. Iyanagi, T. Nakamura, and I. Yamazaki. "One-Electron Transfer Reactions in Biochemical Systems: Analysis of the Reaction Mechanism of Liver Microsomal Vitamin K Epoxide Reductase." Biochemistry 21, (1982): 2986.
Hjerten, Stellan, and Hui Pan. "High-Performance Liquid Chromatography of Proteins on Agarose Columns: Hydrophobic Interaction Chromatography." Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 758, (1983): 49.
Fiske, Cyrus H., and Yoshimasa Subbarow. "The Colorimetric Determination of Phosphorus." Journal of Biological Chemistry 66, (1925), 375.
Banroft JD and Cook BC. Manual of Histological Techniques. Churchill Livingstone, 49.
Chomczynski, P. and Sacchi, N. 'Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction', Analytical Biochemistry, 162(1987), pp. 156-159.
Brown K, Toker A, McNiven MA. Preparation of tissue lysates for Western blot analysis. J Biol Methods;7(2020),140.
Asejeje, F. O., Gabriel, G. O., & Abiola, M. A. Monosodium glutamate aggravates lipopolysaccharide-induced liver injury via inflammation and oxidative stress in rats. Nutrire, 48(2023), 5.
Han, J., Pan, X.-Y., Xu, Y., Xiao, Y., An, Y., Tie, L… Li, X.-J. Curcumin induces autophagy to protect vascular endothelial cell survival from oxidative stress damage. Autophagy, 8(2012), 812.
Xie, T., Chen, X., Chen, W., Huang, S., Peng, X., Tian, L., . . . Huang, Y.. Curcumin is a potential adjuvant to alleviates diabetic retinal injury via reducing oxidative stress and maintaining Nrf2 pathway homeostasis. Frontiers in Pharmacology, 12(2021), 796565.
Shukry, M., El-Shehawi, A. M., El-Kholy, W. M., Elsisy, R. A., Hamoda, H. S., Tohamy, H. G., Farrag, F. A. Ameliorative effect of graviola (Annona muricata) on mono sodium glutamate-induced hepatic injury in rats: Antioxidant, apoptotic, anti-inflammatory, lipogenesis markers, and histopathological studies. Animals, 10(2020), 1996.
Mironczuk-Chodakowska, I., Witkowska, A. M., & Zujko, M. E. Endogenous non-enzymatic antioxidants in the human body. Advances in Medical Sciences, 63(2018), 68.
Al-Kadi, A., Ahmed, A.-S., El-Tahawy, N. F. G., Khalifa, M. M. A., & El-Daly, M. (2020). Silymarin protects against sepsis-induced acute liver and kidney injury via anti-inflammatory and antioxidant mechanisms in the rat. Journal of advanced Biomedical and Pharmaceutical Sciences, 3(2020), 190.
Hewlings, S. J., & Kalman, D. S. (2017). Curcumin: A review of its effects on human health. Foods, 6(2017), 92.
Zhang, M., An, C., Gao, Y., Leak, R. K., Chen, J., & Zhang, F. Emerging roles of Nrf2 and phase II antioxidant enzymes in neuroprotection. Progress in Neurobiology, 100 (2013), 30.
Jinap, S., & Hajeb, P. (2010). Glutamate. Its applications in food and contribution to health. Appetite, 55(1), 1-10.
DOI: https://www.doi.org/10.53766/AcBio/Se encuentra actualmente indizada en: | |||
 |  |  |  |
  |  |  |  |
 |  |  |  |
 |  | ||
Todos los documentos publicados en esta revista se distribuyen bajo una
Licencia Creative Commons Atribución -No Comercial- Compartir Igual 4.0 Internacional.
Por lo que el envío, procesamiento y publicación de artículos en la revista es totalmente gratuito.