Evaluación comparativa de la eficacia antimicrobiana de Momordica charantia y ajo (Allium sativum) como irrigantes endodónticos contra E. faecalis: un estudio in vitro

Anusha Yalamanchi, N. Raghunathan, Snigdha Priya Gopinagaruri, P. Shobana, E. Sri Satyavathi, Reddy E Sujayeendranatha

Resumen


Para evaluar la eficacia antimicrobiana de dos extractos herbales, es decir, Bitter Guard (Momordica charantia) y Ajo (Allium sativum) como irrigantes endodóncicos contra Enterococcus faecalis. Se seleccionaron para el estudio premolares mandibulares humanos unirradiculares extraídos por razones de ortodoncia. Los dientes fueron descoronadizos para estandarizar la longitud a 12 ± 1 mm. La limpieza y conformación de los conductos radiculares se realizó mediante la técnica de corona hacia abajo utilizando limas rotatorias universales protaper hasta F3. Las muestras se colocaron en recipientes de acero que contenían caldo BHI y se esterilizaron en autoclave. A partir de un cultivo madre de la cepa MTCC 2527 de E. faecalis, se realizó un subcultivo en una placa de Agar de prueba de sensibilidad diagnóstica. La enumeración de bacterias vivas (UFC) se realizó mediante el método de dilución seriada. Los conductos radiculares se inocularon con suspensión de E. faecalis y se incubaron a 37 °C durante 21 días. Las muestras se dividieron en cinco grupos, cada uno con diez dientes. Las soluciones de irrigación de prueba se utilizaron de la siguiente manera. Grupo 1: solución salina normal, Grupo 2: NaOCl al 5,25 %, Grupo 3: CHX al 2 %, Grupo 4: Bitter Guard, Grupo 5: ajo. Las virutas dentinarias se recogieron sin una lima de 40 H en condiciones asépticas. Después de la incubación, se contaron las colonias. La media de UFC de baja a alta con todos los irrigantes probados fue la siguiente: Grupo 2: NaOCl al 5,25 % (0,00); Grupo 3: clorhexidina al 2 % (1,14 x 10 -3); Grupo 4: Bitter Guard (1,40 x 10 -3); Grupo 5: ajo (10,30 x 10 -3) y Grupo 1: solución salina normal (28,60 x 10 -3). Los extractos de Bitter Guard (Momordica charantia) y Ajo (Allium sativum) son eficaces contra E. faecalis. Bitter Guard (Momordica charantia) y clorhexidina al 2% son igualmente eficaces contra E. faecalis. El NaOCl al 5,25% demostró una inhibición completa de E. faecalis y se convirtió en el irrigante endodóntico de referencia.

Palabras clave


Momordica charantia; Allium sativum; E. faecalis.

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Referencias


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